Since emergence of
SARS-CoV-2 in late 2019, there has been a critical need to understand
prevalence,
transmission patterns, to calculate the
burden of disease and
case fatality rates.
Molecular diagnostics, the
gold standard for identifying viremic cases, are not ideal for determining true case counts and rates of
asymptomatic infection. Serological
detection of
SARS-CoV-2 specific
antibodies can contribute to filling these
knowledge gaps. In this study, we describe
optimization and validation of a
SARS-CoV-2-specific-
enzyme linked immunosorbent assay (
ELISA) using the prefusion-stabilized form of the spike
protein [1]. We performed receiver operator characteristic (ROC) analyses to define the specificities and sensitivities of the optimized assay and examined cross reactivity with
immune sera from
persons confirmed to have had
infections with other
coronaviruses. These assays
will be used to perform contact investigations and to conduct large-scale, cross sectional
surveillance to define
disease burden in the
population.