The emergence of
SARS-CoV-2 and the ensuing
explosive epidemic of COVID19
disease has generated a need for assays to rapidly and conveniently
measure the
antiviral activity of
SARS-CoV-2-specific
antibodies. Here, we describe a collection of approaches based on
SARS-CoV-2 spike-pseudotyped, single-cycle, replication-defective
human immunodeficiency virus type-1 (
HIV-1) and
vesicular stomatitis virus (VSV), as well as a replication-competent VSV/
SARS-CoV-2 chimeric
virus. While each surrogate
virus exhibited subtle differences in the
sensitivity with which neutralizing activity was detected, the neutralizing activity of both convalescent
plasma and
human monoclonal antibodies measured using each
virus correlated quantitatively with neutralizing activity measured using an authentic
SARS-CoV-2 neutralization assay. The assays described herein are adaptable to high throughput and are useful tools in the evaluation of serologic
immunity conferred by
vaccination or prior SARS-CoV-2 infection, as well as the
potency of convalescent
plasma or
human monoclonal antibodies.