A single-component luminescent biosensor for the SARS-CoV-2 spike protein
Matthew Ravalin; Heegwang Roh; Rahul Suryawanshi; G. Renuka Kumar; John E. Pak; Melanie Ott; Alice Y Ting.
Preprint
en Inglés
| PREPRINT-BIORXIV | ID: ppbiorxiv-496006
Many existing protein detection strategies depend on highly functionalized antibody reagents. A simpler and easier to produce class of detection reagent is highly desirable. We designed a single-component, recombinant, luminescent biosensor that can be expressed in laboratory strains of E. coli and S. cerevisiae. This biosensor is deployed in multiple homogenous and immobilized assay formats to detect recombinant SARS-CoV-2 spike antigen and cultured virus. The chemiluminescent signal generated facilitates detection by an un-augmented cell phone camera. Binding Activated Tandem split-enzyme (BAT) biosensors may serve as a useful template for diagnostics and reagents that detect SARS-CoV-2 antigens and other proteins of interest.
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