A rapid and sensitive method to detect SARS-CoV-2 virus using targeted-mass spectrometry
Praveen Singh; Rahul Chakraborty; Robin Marwal; Radhakrishan V S; Akash Kumar Bhaskar; Himanshu Vashisht; Mahesh S Dhar; Shalini Pradhan; Gyan Ranjan; Mohamed Imran; Anurag Raj; Uma Sharma; Priyanka Singh; Hemlata Lall; Meena Dutta; Parth Garg; Arjun Ray; Debasis Dash; Sridhar Sivasubbu; Hema Gogia; Preeti Madan; Sandhya Kabra; Sujeet K Singh; Anurag Agrawal; Partha Rakhit; Pramod Kumar; Shantanu Sengupta.
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| PREPRINT-MEDRXIV | ID: ppmedrxiv-20161836
In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by the novel corona virus SARS-CoV-2 affecting millions of people worldwide. Early diagnosis and isolation is key to contain the rapid spread of the virus. Towards this goal, we report a simple, sensitive and rapid method to detect the virus using a targeted mass spectrometric approach, which can directly detect the presence of virus from naso-oropharyngeal swabs. Using a multiple reaction monitoring we can detect the presence of two peptides specific to SARS-CoV-2 in a 2.3 minute gradient run with 100% specificity and 90.4 % sensitivity when compared to RT-PCR. Importantly, we further show that these peptides could be detected even in the patients who have recovered from the symptoms and have tested negative for the virus by RT-PCR highlighting the sensitivity of the technique. This method has the translational potential of in terms of the rapid diagnostics of symptomatic and asymptomatic COVID-19 and can augment current methods available for diagnosis of SARS-CoV-2.
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