Cov-MS: a community-based template assay for clinical MS-based protein detection in Sars-Cov-2 patients
Bart Van Puyvelde; Katleen Van Uytfanghe; Olivier Tytgat; Laurence Van Oudenhove; Ralf Gabriels; Robbin Bouwmeester; Simon Daled; Tim Van Den Bossche; Pathmanaban Ramasamy; Sigrid Verhelst; Laura De Clerck; Laura Corveleyn; Nathan Debunne; Evelien Wynendaele; Bart De Spiegeleer; Peter Judak; Kris Roels; Laurie De Wilde; Peter Van Eenoo; Tim Reyns; Marc Cherlet; Emmie Dumont; Griet Debyser; Ruben t'Kindt; Koen Sandra; Surya Gupta; Nicolas Drouin; Amy Harms; Thomas Hankemeier; Donald Jones; Pankaj Gupta; Cathy Lane; Said El Ouadi; Jean-Baptiste Vincendet; Nick Morrice; S. Oehrle; Nikunj Tanna; Steve Silvester; Sally Hannam; Florian Sigloch; Andrea Bhangu-Uhlmann; Jan Claereboudt; Morteza Razavi; Norman Leigh Anderson; Sven Degroeve; Lize Cuypers; Christophe Stove; Katrien Lagrou; Geert Antoine Martens; Dieter Deforce; Lennart Martens; Hans Vissers; Maarten Dhaenens.
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| PREPRINT-MEDRXIV | ID: ppmedrxiv-20231688
Rising population density and global mobility are among the reasons why pathogens such as SARS-CoV-2, the virus that causes COVID-19, spread so rapidly across the globe. The policy response to such pandemics will always have to include accurate monitoring of the spread, as this provides one of the few alternatives to total lockdown. However, COVID-19 diagnosis is currently performed almost exclusively by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Although this is efficient, automatable and acceptably cheap, reliance on one type of technology comes with serious caveats, as illustrated by recurring reagent and test shortages. We therefore developed an alternative diagnostic test that detects proteolytically digested SARS-CoV-2 proteins using Mass Spectrometry (MS). We established the Cov-MS consortium, consisting of fifteen academic labs and several industrial partners to increase applicability, accessibility, sensitivity and robustness of this kind of SARS-CoV-2 detection. This in turn gave rise to the Cov-MS Digital Incubator that allows other labs to join the effort, navigate and share their optimizations, and translate the assay into their clinic. As this test relies on viral proteins instead of RNA, it provides an orthogonal and complementary approach to RT-PCR, using other reagents that are relatively inexpensive and widely available, as well as orthogonally skilled personnel and different instruments. Data are available via ProteomeXchange with identifier PXD022550.
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