Optimization of magnetic bead-based nucleic acid extraction for SARS-CoV-2 testing using readily available reagents
Simon Haile; Aidan M Nikiforuk; Pawan K Pandoh; David D.W. Twa; Duane E Smailus; Jason Nguyen; Stephen Pleasance; Angus Wong; Yongjun Zhao; Diane Eisler; Michelle Moksa; Qi Cao; Marcus Wong; Edmund Su; Martin Krzywinski; Jessica M T Nelson; Andrew J Mungall; Frankie Tsang; Leah M Prentice; Agatha Jassem; Amee R Manges; Steven J.M Jones; Robin J Coope; Natalie Prystajecky; Marco A Marra; Mel Krajden; Martin Hirst.
Preprint
en Inglés
| PREPRINT-MEDRXIV | ID: ppmedrxiv-21251364
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity. Article SummaryA scalable, non-proprietary, magnetic bead-based automated nucleic acid extraction protocol optimised for minimum cross-well contamination
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