Mucosal memory T cells in breastmilk are modulated by SARS-CoV-2 mRNA vaccination
Blair Armistead; Yonghou Jiang; Marc Carlson; Emily S Ford; Saumya Jani; John Houck; Xia Wu; Lichen Jing; Tiffany Pecor; Alisa Kachikis; Winnie Yeung; Tina Nguyen; Nana Minkah; Sasha E Larsen; Rhea N Coler; David M Koelle; Whitney E Harrington.
Preprint
en Inglés
| PREPRINT-MEDRXIV | ID: ppmedrxiv-21267036
Human breastmilk is rich in T cells; however, their specificity and function are largely unknown. We compared the phenotype, diversity, and antigen specificity of T cells in the breastmilk and peripheral blood of lactating individuals who received SARS-CoV-2 mRNA vaccination. Relative to blood, breastmilk contained higher frequencies of T effector and central memory populations that expressed mucosal-homing markers. T cell receptor (TCR) sequence overlap was limited between blood and breastmilk. Overabundan t breastmilk clones were observed in all individuals, were diverse, and contained CDR3 sequences with known epitope specificity including to SARS-CoV-2 Spike. Spike-specific TCRs were more frequent in breastmilk compared to blood and expanded in breastmilk following a third mRNA vaccine dose. Our observations indicate that the lactating breast contains a distinct T cell population that can be modulated by maternal vaccination with potential implications for infant passive protection. One-Sentence SummaryThe breastmilk T cell repertoire is distinct and enriched for SARS-CoV-2 Spike-specificity after maternal mRNA vaccination.
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