The study was conducted in St. John's Medical College
Hospital and Department of
Physiology, with the aim of studying the
serum nitrate levels in
pre-eclampsia and normal
pregnancy. The total number of subjects studied in various groups were 159, control (n = 55),
first trimester (n = 13),
second trimester normal (n = 42),
second trimester pre-eclampsia/
PET (n = 5),
third trimester normal (n = 32),
third trimester pre-eclampsia/
PET (n = 12). The
serum nitrate was measured by one step
enzymatic assay using
Nitrate reductase from
Aspergillus species. The
nitrate levels in the
third trimester pre-eclamptic group was found to be significant lower (P = 0.02), as compared to normal subjects, however the renal functions were normal in all the subjects.