Background: Phospholipase A2 (PLA2) is involved in
inflammation and
cell death following
stroke , and inhibition of its activity may promote neuroregeneration. This study aimed to observe the influence of
Acalypha indica Linn root extract towards relative
cell viability and PLA2
enzyme level in post-hypoxic hippocampal
tissue culture .
Methods: Experimental
in vitro study using 24 primary neuronal
cell cultures obtained from
Sprague Dawley rat exposed to
hypoxia with 5% O2 / 5% CO2 / N2 balanced gas for 24 hours. Post-
hypoxia ,
Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to three
treatment groups. No
treatment was given to the
control group . Each group consists of six samples. After 72 hours of incubation, relative
cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) examination, and
phospholipase A2 enzyme level was determined using
ELISA .
Results: PLA2
enzyme level of
rat hippocampal
tissue culture treated with
Acalypha indica Linn root extract
at 10 , 15, and 20 mg/mL were significantly lower than that of control (5.55 ng/mL, 6.85 ng/mL, and 7.42 ng/mL vs 7.96 ng/mL, p < 0.05).
Conclusion: Acalypha indica Linn root extract increases the relative
cell viability and decreases the PLA2
enzyme level of post-hypoxic
mouse hippocampal
tissue with the optimal
dose of the extract
at 10 mg/mL.