The aims of this study are the investigation of the effects of
fibronectin and
type IV collagen extracellular matrix proteins and the
role of
caspase-3 and -9 on cis-platin induced U2-OS
apoptosis were studied. First the cytotoxic effects of cis-platin on
cell system were investigated by colorimetric
method and than morphological and
ELISA analysis were used for
determination of
cell apoptosis when induced with cis-platin. In addition, after adhering the
cells to fibronection or
type IV collagen proteins, the apoptotic rate and the effects of
caspase-3 and -9 were also investigated by
ELISA in presence of specific inhibitors. U2-OS
cells showed 20% cytotoxicity
after treatment with 2.4 µM of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced
apoptosis on
cells as a
dose-dependent manner.
Caspase-3 and -9 inhibitors inhibited cis-platin-induced
apoptosis in U2-OS
cells, respectively. The binding of
cells to 10 µg/mL of
fibronectin but not
type IV collagen enhanced the
apoptosis about 2.5 fold that effects inhibited with
caspase-3 inhibitor. The
caspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to
fibronectin, but not
type IV collagen enhanced the apoptotic response of U2-OS and
fibronectin-dependent
apoptosis was activated by
caspase-3. These finding might be useful for
patients to fight against
osteosarcoma.