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Background:

Diabetic subjects, especially women, show high prevalence of asymptomatic bacteriuria (ASB). The aim of the present study was to evaluate the prevalence of asymptomatic bacteriuria (ASB) in patients with type 2 diabetes mellitus and to find out the antibiotic sensitivity pattern of bacterial isolates.

Methods:

One hundred and thirty type 2 diabetics comprising 56 males and 74 females (aged between 30 - 59 years) attending the outpatient department of Chhattisgarh Institute of Medical sciences hospital, Chhattisgarh, India was recruited in the study. Mid-stream urines were collected from patients aseptically into sterile wide mouth container and examined microscopically, culturally using standard techniques and tested for glucose, post-prandial glucose, protein and ketone using a dipstick. Samples were cultured on blood agar, McConkey agar and Cysteine Lactose Electrolyte Deficient (CLED) media and incubated at 37°C aerobically for 24 h. Isolates were tested against antibiotics which included tetracycline, chloramphenicol, ciprofloxacin and cotrimoxazole by the disc diffusion method.

Results:

Significant bacteriuria was observed in Forty-seven (36.15%) of urine samples (34 females and 13 males) . Bacteria isolated included Escherichia coli (56.9%), Enterobacter sp. (12.7%), Klebsiella pneumoniae (8.5%) and Proteus sp. (6.3%). E. coli, Enterobacter sp. K. pneumoniae, S. aureus and Proteus sp. were most sensitive to cotrimazaxole, amoxicillin, nalidixic acid and ciprofloxacin but a large number of bacteria were resistant to tetracycline, chloramphenicol and ampicillin.

Conclusion:

Screening for ASB is necessary in diabetic patients especially if pyuria is detected in urine analysis since ASB has been found to be a risk factor for developing symptomatic urinary tract infection.