Development and evaluation of flavi-immunoglobulinM capture enzyme-linked immunosorbent assay.
Palani, Gunasekaran; Padmanabhan, Padma Priya; Ramesh, Kiruba; Asadullah, Khaleefathullah Sheriff; Sambasivam, Mohana; Arunagiri, Kavita; Krishnasamy, Kaveri.
Indian J Pathol Microbiol
; 2013 Jul-Sept 56 (3): 269-271
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| IMSEAR | ID: sea-155882
In this study, we report the evaluation of In-house fl avi virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA), which can be used as a screening test to determine the infecting fl avivirus serotype over the current serological methods. A panel of 88 sera (inclusive of well characterized dengue, Japanese Encephalitis (JE) and West Nile virus (WNV) positive and negative samples tested and confi rmed by commercial kit) was used for evaluation of the kit. The sensitivity and specifi city of the In-house capture assay versus the commercial kit for the sero-diagnosis of dengue was 100% and 87% respectively, for JE IgM, it was found to be 90% and 100% respectively, and for West Nile it was 87.5% and 90.9%. Based on the study, we concluded that this fl avivirus-serotyping ELISA provides rapid results and may be used as an accurate alternate to other serological tests for the specifi c diagnosis of fl avivirus infections.
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