Mature wheat grain contain 8-20% proteins. The storage proteins of wheat determine the characteristics and unique properties of its flour in the process of pasta and bread-making and eventually the quality of the finished products obtained. The storage proteinsplay an integral role in determining the visco-elastic properties of wheat dough, a feature of high quality wheat. The genes, encoding storage proteins as well as factors that may affect their deposition, such as molecular chaperones and foldase enzymes, are of particular interest to wheatindustry. Genomic, cDNA and promoter sequences of the three homoeologous gene encoding the “typical” PDI has been already cloned and characterized. Recently, eight new non-homoeologous wheatgenes were cloned and characterized in breadwheat. Study characterizing the variability in a 700 bp region comprising 600 bp of 5’ upstream putative promoter region and 100 bp of the first exon of the typical PDI gene in 15 accessions of Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops taushcii (DD), from diverse origin and Triticum aestivum cv Chinese Spring (AABBDD) has also been completed recently. This review explains how functional and evolutionary genomics tools aid in the study of a genefamily and its promoter.