Compare some kinds of protein extraction methods and find the best one among them suitable to tomatoflower buds. Place and Duration of Study Biological Science and Technology College, between June 2010 and July 2011.
Few spots were found by SDS and Phenol/SDS extractions, more spots by immediate dissolution but the most impurities, less proteinproductivity though more spots by Tris-Phen extractions, and more proteinproductivity and better apart effect by TCA/acetone. The 2-DE image background was the clear and the protein spots were the most by TCA/acetonemethod.