The search for novel
antibiotics is of immense importance in
research areas around the world for agricultural,
pharmaceutical , and industrial applications. The
Streptomyces species are widely used as an importantbiological tool for the
production of a wide range of novel
secondary metabolites. In the present study, isolatedstrain RSA-14 from
rhizosphere soil of Alternanthera sessilis was subjected to morphological, physiological,biochemical and
16S rRNA gene sequence analysis . The isolated RSA-14 was analyzed for antimicrobialactivities by cross streak
method and exhibited a broad spectrum of antimicrobial activity against test pathogens.The isolate was tested for the
ability to grow in the presence of
antibiotics , such as
penicillin ,
streptomycin ,
chloramphenicol ,
gentamycin , and
tetracycline and resistant to only two
antibiotics , and sensitive to others. The16S
ribosomal RNA gene sequencing and
analysis of the
phylogenetic tree showed 100% sequence similaritywith
Streptomyces cinereoruber
strain P.B.373.