ELISA for detection of dengue virus-induced cytokine and its antibody.
Mukerjee, R; Chaturvedi, U C.
Indian J Exp Biol
; 1997 Mar; 35(3): 225-31
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| IMSEAR | ID: sea-59794
ELISA technique has been standardized for detection of a dengue type 2 virus (DV)-induced cytokine, the cytotoxic factor (CF) and CF-specific antibodies. The performed ELISA was found to be sensitive (90.9%), specific (92.5%), accurate (91%) and reproducible and was able to detect a minimum of 7 ng/ml CF in the test samples. The technique was used to detect CF in DV inoculated mouse sera and DV-infected mouse spleen cell culture fluids. Significant utility of the test was detection of a CF-like cytokine in the sera of human cases of dengue haemorrhagic fever.
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