BACKGROUND:
Recently
atherosclerosis and
coronary artery disease (CAD) are considered to be inflammatory
diseases. The
genetic polymorphism in inflammatory markers has been well studied and found to be associated with development of CAD.
AIM:
To study the
association of biallelic polymorphism at position 196 in
exon 6 of
tumor necrosis factor 2 (
TNFR2)
gene and
coronary artery disease. SETTINGS AND
DESIGN:
The study design was a prospective
case control study conducted at a
tertiary referral center mainly catering to the north Indian
population. MATERIALS AND
METHODS:
One hundred and fifty angiographically proven
patients with
coronary artery disease and one hundred and fifty age matched controls were genotyped for
TNFR2 gene by
polymerase chain reaction followed by
analysis of
restriction fragment length polymorphism. STATISTICAL
ANALYSIS:
Genotype frequencies were compared in
patients and controls by
Chi-square test. Binary
logistic regression analysis was used to examine the relationship between
genotypes and
disease, incorporating other variables into the model.
RESULTS:
The
incidence of CAD in those with MM
genotype was 65% and in those with RM
genotype was 42%.
Genotype frequency shows significant
association of MM
genotype with development of CAD (P < 0.001;
odds ratio-2.585; 95%
confidence interval 1.533-4.359). The
association of
TNFR2 genotype with CAD persisted on
logistic regression analysis.
CONCLUSION:
MM
genotype of
TNFR2 gene is associated with development of CAD and RM
genotype appears to be protective.