Study of membrane potential in T lymphocytes subpopulations using flow cytometry
Queiroz, Fernanda Mello de; Ponte, Cristiano G; Bonomo, Adriana; Jorge, Rosane Vianna; Suarez-Kurtz, Guilherme.
BMC Immunol
; 92008. ilus
Artículo
en Inglés
| TXTC | ID: txt-24820
Abstract Ion channels are involved in the control of membrane potential () in a variety ofcells. The maintenance of in human T lymphocytes is essential for T-cell activation and wassuggested to depend mostly on the voltage-gated Kv1.3 channel. Blockage of Kv1.3 inhibits cytokineproduction and lymphocyte proliferation in vitro and suppresses immune response in vivo. Tlymphocytes are a heterogeneous cell population and the expression of Kv1.3 varies among cellsubsets. Oxonol diBA-C4-(3) was used to determine by flow cytometry. The presence of distinctT cell subsets was evaluated by immunophenotyping techniques and the contribution of Kv1.3channels for the maintenance of was investigated using selective blockers.
Results:
The distribution of in T lymphocytes varied among blood donors and did not alwaysfollow a unimodal pattern. T lymphocytes were divided into CD3+/CD45RO- and CD3+/CD45RO+subsets, whose peak channel values of were -58 ñ 3.6 mV and -37 ñ 4.1 mV, respectively. MgTX(specific inhibitor of Kv1.3 channels) had no significant effect in the of CD3+/CD45RO- subsetsbut depolarized CD3+/CD45RO+ cells to -27 ñ 5.1 mV.Conclusion:
Combination of optical methods for determination of by flow cytometry withimmuophenotyping techniques opens new possibilities for the study of ion channels in the biologyof heterogeneous cell populations such as T lymphocyte subsets.(AU)
Biblioteca responsable:
BR440.1
Ubicación: BR440.1
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