To evaluate the effect of
melatonin on
in vitro maturation of
goat oocytes, 12
females were used asoocyte
donor. They were subjected to
ovarian stimulation and
ovum pickup by
laparotomy.
Oocytes wereevaluated and allocated into three groups of
in vitro maturation M0 (
oocytes matured in TCM199 andsupplements); M10 and M50 (
oocytes matured in M0 medium supplemented with 10 μM and 50 μM
melatonin,respectively). All groups were subjected to the
cell incubator for 24 hours at 38.5°C with a humidifiedatmosphere containing 5% CO2. For
data analysis,
Chi-square test was used, with a
probability of 5% and acorrelation test. In conclusion,
melatonin reduced
in vitro maturation rates of
goat oocytes.(AU)