The Saanen
goat breed has been widely explored in
breeding programmes; however, there are few
reports about the breeds genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of
spermatozoa from Saanen
breeding goats. Five
breeding animals with proven
fertility were selected, the
spermatozoa were collected, and the
protein was extracted. Subsequently, the
proteins were
separated and analysed by two-dimensional
electrophoresis and
mass spectrometry; the
proteins were then identified with the
SwissProt database. A total of 31
proteins involved in
reproduction were identified, including
binding proteins on
spermatozoa for fusion with the
egg, acrosomal
membrane proteins, metabolic
enzymes,
heat shock proteins,
cytoskeletal proteins and
spermatozoa motility proteins. The characterization of such
proteins clarifies the molecular mechanisms of
spermatogenesis and the modifications that ensure the success of
fertilization.(AU)