Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niterói, Rio de Janeiro / Avaliação molecular de piroplasmídeos e alterações hematológicas em amostras de sangue de cães armazenadas em laboratório de análises clínicas de Niterói, Rio de Janeiro
Santos, Fernanda Barbosa dos; Gazeta, Gilberto Salles; Correa, Laís Lisboa; Lobão, Lucas Fernandes; Palmer, João Pedro; Dib, Laís Verdan; Damasceno, José André Lessa; Moura-Martiniano, Nicole Oliveira; Bastos, Otilio Machado Pereira; Uchôa, Claudia Maria Antunes; Barbosa, Alynne da Silva.
R. bras. Parasitol. Vet.
; 29(3): e012420, 2020. graf, tab
Artículo
en Inglés
| VETINDEX | ID: vti-27294
Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.(AU)
Biblioteca responsable:
BR68.1
Ubicación: BR68.1
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