Detecção molecular de vírus da bronquite infecciosa em plantéis de avós, matrizes e frangos de corte no Rio Grande do Sul e Mato Grosso
Nascimento, Bárbara; Comerlato, Juliana; Rosado Spilki, Fernando.
Ci. Rural
; 43(3)2013.
Artículo
en Portugués
| VETINDEX | ID: vti-708558
Infectious bronchitis virus (IBV, Avian Coronavirus) from chickens belongs to group 3 of the family Coronaviridae and causes respiratory and renal disorders in broilers. Vaccination using live vaccines is generally performed in mothers and grandmothers, as well as often in flocks for slaughter. The vaccines used in Brazil are usually from serogroup Massachusetts and based on standard samples of the virus at passages H120 and H52. It is common that after vaccination the vaccine virus is detected by isolation in embryonated eggs or by molecular methods for up to four weeks. After, there is usually no virus detection and any IBV found may represent recirculation of the vaccine virus in the flock or the introduction of a new strain. In this study, to evaluate the circulation of the virus in poultry flocks and breeders in the state of Rio Grande do Sul and Mato Grosso do Sul, 240 samples were collected from tracheas and kidneys of birds from 48 flocks, and (20 biological samples / 4 flocks) from grandmothers (80 samples/16 flocks) and mothers (140 samples/28 flocks) from broilers, which were analyzed in pools of five samples. All animals were vaccinated and samples were collected around 2-48 weeks after vaccination. The presence of IBV was determined with the aid of a polymerase chain reaction "nested" gene-directed protein S1, standardized in this study. From the 48 samples tested, 14 were positive 5 were from birds vaccinated after less than 4 weeks and 9 were from birds vaccinated more than four weeks should be wild viruses or represent the recirculation of the vaccine virus.
Biblioteca responsable:
BR68.1
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