Endometrial prostaglandin F2 in vitro production and its modulation regarding dominant follicle position in cattle / Produção in vitro de PGF F2 endometrial e sua modulação referente ao folículo dominante em bovinos
Fernandes Bressan, Fabiana; Maria Bertan Membrive, Claudia; Demarchi Goissis, Marcelo; Belentani Marques, Vanessa; Martins da Cunha, Pauline; Binelli, Mario.
Braz. j. vet. res. anim. sci
; 55(2)2018.
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en Inglés
| VETINDEX | ID: vti-732851
Prostaglandin F2 (PGF2) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2 due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2 stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2 content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2 content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P 0.01). However, the PGF2 levels did not differ between the UHs as a function of t
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