Abstract
Detection of
Salmonella is very important to minimize the
food safety risk. In this study, the recombinant PagC
protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture
Salmonella cells from
pork and
milk samples. And then the SYBR Green qualitative
PCR was developed to detect the pathogenic
Salmonella. The results showed that the PagC polyclonal antiserum is of good
specificity and the capture rate of 0.1 mg IMBs for
Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The
method developed demonstrated high
specificity for the positive
Salmonella samples when compared to non-specific
DNA samples, such as
Escherichia coli,
Staphylococcus aureus,
Yersinia enterocolitica, and
Yersinia pseudotuberculosis. The
limit of detection of this assay was 18 CFU/mL.
Detection and quantitative enumeration of
Salmonella in samples of
pork or
milk shows good recoveries of 54.34% and 52.07%. In conclusion, the polyclonal antibody of recombinant PagC
protein is effective to capture
Salmonella from detected samples. The developed pagC antibody IMBs-qPCR
method showed
efficiency,
sensitivity and specificity for 30
Salmonella detection, enabling
detection within 10 h, which is a promising rapid
method to detect
Salmonella in
emergency.