Purpose@#The main
treatment options of
neurogenic bladder remains
catheterization and long-term oral medications. Metabolic interventions have shown good
therapeutic results in many
diseases. To date, no studies have characterized the metabolites of the detrusor
muscle during
neurogenic bladder. Using
metabolomics, new
muscle metabolomic signatures were identified to reveal the temporal
metabolic profile of
muscle during
disease progression. @*
Methods@#We used 42
Sprague-Dawley rats (200±20 g,
males) for T10 segmental
spinal cord injury modeling and collected detrusor
tissue and performed nontargeted
metabolomics after sham
surgery, 30-minute, 6-hour, 12-hour, 24-hour, 5-day, and 2-week postmodelling, to identify the dysregulated
metabolic pathways and key metabolites. @*Results@#By comparing mzCloud, mzVault, MassList, we identified a total of 1,271 metabolites and enriched a total of 12
metabolism-related pathways with significant differences (P<0.05) based on Kyoto
Encyclopedia of
Genes and
Genomes analysis. Metabolites in several differential
metabolic pathways such as ascorbate and aldarate
metabolism,
Steroid hormone biosynthesis, and
carbon metabolism are altered in a regular manner before and after
ridge shock. @*Conclusions@#Our study is the first
time-based
metabolomic study of
rat forced urinary
muscle after traumatic
spinal cord injury, and we identified multiple differential
metabolic pathways during
injury that may improve long-term management
strategies for
neurogenic bladder and reduce
costs in long-term
treatment.