BACKGROUND@#
Interstitial cystitis (IC) is a chronic and intractable
disease that can severely deteriorate
patients’
quality of life. Recently,
stem cell therapy has been introduced as a promising alternative
treatment for IC in
animal models. We aimed to verify the
efficacy and
safety of the
human perirenal
adipose tissue-derived
stromal vascular fraction (SVF) in an IC
rat model. @*
METHODS@#From eight-week-old
female rats, an IC
rat model was established by
subcutaneous injection of 200 lg of uroplakin3A. The SVF was injected into the
bladder submucosal layer of IC
rats, and
pain scale
analysis, awakening cytometry, and histological and
gene analyses of the
bladder were performed. For the in vivo
safety analysis, genomic
DNA purification and histological
analysis were also performed to check tumorigenicity and
thrombus formation. @*RESULTS@#The mean
pain scores in the SVF 20 ll group were significantly lower on days 7 and 14 than those in the
control group, and
bladder intercontraction intervals were significantly improved in the SVF groups in a
dose-dependent manner.
Regeneration of the
bladder epithelium,
basement membrane, and
lamina propria was observed in the SVF group.In the SVF groups, however,
bladder fibrosis and the expression of inflammatory markers were not significantly improved compared to those in the
control group. @*CONCLUSION@#This study demonstrated that a perirenal
adipose tissue-derived SVF is a promising alternative for the management of IC in terms of improving
bladder pain and overactivity.