【Objective】 To explore the identification
method and characteristics of anti-IFC against Cromer
blood group. 【
Methods】
ABO blood group was identified by tube
method, and Rh
blood group was identified by Rh typing card. Irregular antibody
screening and antibody identification were carried out using microtube column
agglutination technology(MCAT). The
serum of the
patient reacted with panel
cells treated with antitrypsin,
trypsin and
bromelain respectively to determine the
specificity of the antibody. The
serum was inhibited with pure CD55
protein for antibody identification. The DAF, the
regulatory gene of Cromer
blood group system, was amplified and sequenced. The expression of CD55 on
cell membrane was analyzed by
flow cytometry. 【Results】 The
patient′s
blood type was B, CcDEe. The
patient′s
serum reacted with all the untreated panel red
cells,
bromelain-treated red
cells, trpsin-treated red
cells, and DTT treated red
cells.It was negative with chymotypsin-treated
cells and could be neutralized by CD55
protein. No
mutation was found by DAF sequencing. The expression of CD55 on
patient′s
cell membrane was deficient. 【Conclusion】 This high frequency antibody was identified as anti-IFC. The
transient depression in CD55
protein may due to the
patient′s GI system
abnormalities.