Agkistrodon acutus is a traditional
Chinese herb
medicine which has immunological
regulation,anti-
tumor,anti-inflammatory and
analgesic effects,which is mainly used for the
treatment of
rheumatoid arthritis,
ankylosing spondylitis,
sjogren's syndrome and
tumors. In order to excavate more important functional
genes from A. acutus,the
transcriptome of the
venom gland was sequenced by the Illumina Hi Seq 4000,and 32 862 unigenes were assembled. Among them,26 589 unigenes were mapped to least one public database. 2 695 unigenes were annotated and assigned to 62 TF
families,and 5 920 SSR loci were identified. The majority of mapped unigenes was from Protobothrops mucrosquamatus in the NR database,which revealed their closest homology. Three secretory
phospholipase A_2 with different
amino acid sequences showed
similar spatial structures and all had well-conserved
active sites. The 3 D
structural models of
C-type lectin showed conserved
glycosylation binding sites( Asn45). This study
will lay the
foundation for the further study of the function of
snake venom protein,and promoting the development and utilization of
genome resources from A. acutus.