Objectives Objectives To investigate how the imbalance of innate
lymphoid cells (ILCs)in the peripheral
blood of
patients with
lung adenocarcinoma affects the balance of
downstream mononuclear
macrophages and T helper (Th)
cells, and to identify the impact of the imbalance of ILCs on the immune status and
prognosis of
lung adenocarcinoma.
Methods The peripheral
blood of 20
patients with
lung adenocarcinoma and normal controls were collected. The percentage of ILCs, mononuclear
macrophages and
T lymphocyte in peripheral
blood were analyzed by
flow cytometry. The characteristic
cytokine secretion levels of various types of immune
cells in peripheral
blood were detected by real-
time fluorescence quantitative
PCR. Results Compared with the normal controls, the proportion of M2 mononuclear
macrophages, ILC1 and ILC2 in
patients with
lung adenocarcinoma was up-regulated, while the proportion of M1 mononuclear
macrophages, CD4+ T and CD8+ T was down-regulated. The
mRNA expression of related
cytokines of M1 mononuclear
macrophages and ILC1 were decreased; while the
mRNA expression of related
cytokines of M2 mononuclear
macrophages and ILC2 were increased. Along with the decreased CD4+
T cells-associated
cytokine T-bet
mRNA expression, and the increased GATA3
mRNA expression. Moreover, the expression of PD-1 in CD8+
T cells was also up-regulated. Conclusion The imbalance of ILCs in peripheral
blood of
patients with
lung adenocarcinoma promotes the imbalance of mononuclear
macrophages and Th
cells, which altogether maintains the
immunosuppression in
patients with
lung adenocarcinoma, and promotes the development of
lung adenocarcinoma.