In the present study, we examined the effects of Dendropanax morbifera Léveille leaf extract (DML) on
D-galactose-induced morphological changes in
microglia and
cytokines, including pro-inflammatory
cytokines (
interleukin [IL]-1β,
IL-6, and
tumor necrosis factor [TNF]-α) and anti-inflammatory
cytokines (
IL-4 and
IL-10) in the
hippocampus.
Administration of DML to
D-galactose-treated
mice significantly improved
D-galactose-induced reduction in escape latency,
swimming speed, and spatial preference for the target quadrant. In addition,
administration of DML to
D-galactose-treated
mice significantly ameliorated the microglial activation and increases of
IL-1β,
IL-6, and TNF-α levels in the
hippocampus.
Administration of
D-galactose significantly reduced
IL-4 levels in the
hippocampus, while
administration of DML to
D-galactose-treated
mice significantly increased
IL-4 level. However, we did not observe any significant changes in
IL-10 levels in hippocampal homogenates. These results suggest that DML reduces
D-galactose-induced
mouse senescence by reducing pro-inflammatory
cytokines such as
IL-1β,
IL-6, and TNF-α, as well as increasing anti-inflammatory
cytokine IL-4.