Aim To explore the effect of four
stilbenes including rhaponticin, desoxyrhaponticin, rhapontigenin and
resveratrol on
glucose and
lipid metabolism in
insulin-resistant
HepG2 cells induced by high
glucose and high fat.
Methods The model of
insulin resistance was established by incubating
HepG2 cells with a complex of
glucose and
oleic acid. MTT assay was used to detect
cell viability. The intracellular
triglyceride (TG) and
glucose levels were measured by the kit
method. The
lipid production was observed by oil red O
staining, and the
cell morphology and uptake of 2-NBDG were observed by confocal microscope. The
PPAR signaling pathway and PI3K/Akt
insulin signaling pathway related
proteins were determined by
Western blot to evaluate the effect of
stilbenes on
glycolipid metabolism in IR-
HepG2 cells. Results The complex containing 50 mmol • L