Aim To explore the mechanism of
Danshen decoction in the
treatment of
colon cancer using
network pharmacology and
molecular docking.
Methods The active components and corresponding target
proteins of
Salvia miltiorrhiza,
Santalum album and Amo-mum villosum in
Danshen decoction were screened based on
Traditional Chinese Medicine Systems Pharmacology database and
analysis platform.The targets of
colon cancer were searched by using Genecards database, and the common targets were selected.The network diagram of
traditional Chinese medicine-active components-target-
disease was constructed by using Cytoscape 3.7.0.The
protein protein interaction network of common targets was constructed by using STRING database.The
gene ontology(GO)and Kyoto
Encyclopedia of
Genes and Gnomes(KEGG)enrichment
analysis were carried out based on R4.0.2.The important targets in the key pathways and the important active components in the network diagram of
traditional Chinese medicine-active ingredients-target-
disease network were selected for Surflex Dock.Results A total of 78 active components, 142 targets, 3 239
colon cancer targets, 105 overlapping targets and 69 corresponding active components were screened out.KEGG
analysis showed that the key signaling pathway was PI3K/AKT.
Luteolin and Tanshinone IIA with high correlation were selected to dock with
protein kinase B(AKT1).Both active components had
hydrogen bonding with AKT1.Conclusions
Danshen decoction
plays a positive
role in
colon cancer treatment.The mechanism may be related to the
regulation of PI3K/AKT signaling pathway.