Paraquat (1,1'-dimethy-4,4'-bipyridinium dichloride, PQ) is an effective and widely used herbicide, which was introduced commercially in 1962. It is reduced by an electrondonor, such as NADPH, and then transfers the electron to molecular oxygen. As a result, the reactive oxygen species (ROS) produced are related to its cellular toxicity. Resveratrol (trans-3,4',5-trihydroxystilbene), a naturally occurring hydroxystilbene, is considered an essential antioxidative constituent of red wine, possessing chemopreventive properties. However, the influence of resveratrol on PQ-induced oxidative cell damage has not fully been investigated.
According to results of the MTT and LDH assays, incubation of cultured lungcells with resveratrol did not protect lungcells from PQ-induced cytotoxicity, and no decrease in ROS production was observed, according to results of the DCF-DA assay. On the other hand, incubation of lungcells with non-lethal resveratrol resulted in aggravation of PQ-induced oxidative stress.
CONCLUSION:
Results of this study showed that incubation of cells with resveratol did not result in reduction of PQ toxicity, but lead to elevation of PQ-induced oxidative stress in cultured lungcells.