Among the 195 specimens, 17 (8.7%) were M. pneumoniae positive, and 3 of the strains (17.6%) obtained from these 17 specimens displayed the A2063G mutation in 23S rRNA. Three macrolide-resistant M. pneumoniae isolates were isolated from patients hospitalized at the primary carehospital. The positive rates of M. pneumoniae for the primary and tertiary carehospitals were 12.1% (15/124) and 2.8% (2/71), respectively (P=0.033).