Preparation and identification of the oligonucleotide chip for detecting the virulence-associated genotypes and drug resistance of Helicobacter pylori / 中国医学科学院学报
Shu-Bin GUO; Jun-Bo LIU; Lin-Jie CHEN; Sheng-Qi WANG.
Acta Academiae Medicinae Sinicae
; (6): 98-102, 2007.
Artículo
en Zh
| WPRIM | ID: wpr-230024
<p><b>OBJECTIVE</b>To develop an oligonucleotide microarray-based method for the simultaneous detection of Helicobacter pylori (Hp) infection, virulence-associated genotypes, and drug resistance.</p><p><b>METHODS</b>Hp was classified into cagA + and cagA- genotypes based on its virulence. Clarithromycin-resistance of Hp was identified by existence of point mutations in 23S rRNA. We constructed an oligonucleotide microarray chip to simultaneously diagnose Hp infection and detect its virulence-associated genotypes and mutations associated with clarithromycin-resistance. The diagnostic accuracy of the constructed microarray was tested with templates of wild type and mutated type.</p><p><b>RESULTS</b>The oligonucleotide chip accurately detected cagA + and cagA- genotypes of Hp, as well as four common point mutations in 23S rRNA related to clarithromycin-resistance.</p><p><b>CONCLUSION</b>Oligonucleotide microarray chip can be used to diagnose Hp infection and test its virulence-associated genotypes and drug resistance simultaneously.</p>
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