The aim of this study was to assess the expressions of CD44 and CD133 in colorectal cancertissue by using immunohistochemical staining and to analyze the clinical significance of the expressions related to other clinicopathological data and survival results.
METHODS:
One hundred sixty-two patients with a biopsy-proven colorectal adenocarcinomawho were operated on between January 1998 and August 2004 were enrolled in this study. Immunohistochemical staining for CD44 and CD133 was performed on primary colorectal cancertissue, metastatic lymph nodes, and synchronous and metachronous metastatic tumortissues if available.
RESULTS:
CD44 expression was stronger in the primary tumor than in metastatic lymph nodes (P < 0.001), and CD133 expression tended to be stronger in primary tumor than in metastatic lymph nodes (P = 0.057). No significant correlation was found between the CD44 and the CD133 expressions. The cases with recurrence showed low expression of CD44 (P = 0.017). CD133 expression was lower in cases with elevated CA 19-9 serum levels (P = 0.028) and advanced T stage (P = 0.038). Multivariate analysis proved that low expression of CD44 was an independent prognosis factor for short disease-free survival (P = 0.028).
CONCLUSION:
Low CD44 expression was correlated with increased tumorrecurrence and short disease-free survival, and low CD133 expression was associated with advanced tumor stage. We suggest that further studies be performed to evaluate whether the immunohistochemical method for determining the CD44 and the CD133 expressions is appropriate for exploring cancerstem-cell biology in patients with colorectal cancer.