Objective To understand the phenotypic characteristics of foodbome
Vibrio parahaemolyticus in Guangdong province through
carrying out a comprehensive comparison including
pulse field gel
electrophoresis,
ribotyping and
serotyping.
Methods 74 different
Vibrio parahaemolyticus strains isolated from
seafood and cases due to
food poisoning in Guangdong province were under
serotyping and susceptibility testing,in addition to the testing of direct
heat hemolysin(tdh)and the
heat hemolysin-related
hemolysin hormone(trh)via
PCR.Ribosomal genotyping(
ribotyping)with EcoR Ⅰ restriction
enzyme was utilized on 74 different
Vibrio parahaemolyticus isolates,whereas
pulsed-field gel electrophoresis(PFGE)with the Not Ⅰ restriction
enzyme was used on 74 different
Vibrio parahaemolyticus isolates.BioNumerics
software was used to compare the isolates from different sources,times and places in order to elicit the correlation between different
strains.Results Although
Vibrio parahaemolyticus was 100.00% sensitive to
chloramphenicol,it still presented different levels of resistance against 13 other
antibiotics.Among the 74 different
strains of
Vibrio parahaemolyticus under testing,24.32% showed positive for the tdh
virulence gene,whereas 4.05% positive for trh.74 different
Vibrio parahaemolyticus strains were found to belong to 26
serotypes,where the O5K17 and O2K28
serotypes were dominant in those isolates that causing
seafood-
poisoning.The O3K6
serotype was found to be the dominant of those isolates that causing
food-
poisoning.Based on ribosomal genotyping,the 74
Vibrio parahaemolyticus isolates were divided into 62 different
ribotypes,whereas the 74
strains of
Vibrio parahaemolyticus were divided into 67 different PFGE types,thus exhibiting considerable
genetic diversities of the
strains.Conclusion Majority of the isolates causing
food-
poisoning carried tdh
virulence gene.PFGE was shown to have the highest resolution,followed by
ribotyping with
serotyping being the lowest,where the combination of the three could improve the resolution.