Objective To study the
serotypes,
virulence features and molecular characteristics of
Vibrio parahaemaolyticus isolated in
food poisoning cases and
surveillance program on
diarrhea patients in Guangdong,2009.
Methods 95
Vibrio parahaemolyticus strains from
food poisoning cases and 15
strains from
surveillance program on
diarrhea patients were serotyped and detected for tdh(thermostable direct
hemolysin,tdh)and trh(tdh-related
hemolysin gene,trh)by
PCR.81 sero-variant
Vibrio parahaemolyticus strains were selected through PFGE subtyping.Results There were 15
Vibrio parahaemolyticus strains isolated from
surveillance program on
diarrhea patients and 95
strains were isolated from 11
Vibrio parahaemolyticus-caused
food poisoning cases in 2009.Among these
strains,O3K6(46.67% and 44.21%)and O4K8(33.33% and 28.42%)were the dominant
serotypes,but not the 7
food-borne
strains.There were 93(84.54%)tdh +trh-,13(11.81%)tdh-trh-,and 3(3.65%)tdh+ trh +
strains.The similarity value was between 57.7% to 100.0% of the 81
strains after PFGE sub-typing
method and 36 PFGE subtypes were identified.PFGE001 and PFGE029 appeared to be the dominant subtypes.Conclusion O3K6 and O4K8 were the most dominant
serotypes in
Vibrio parahaemolyticus-caused
diarrhea and
food poisoning cases in Guangdong and tdh were detected in most of the
strains.Dominant PFGE subtypes were causing both sporadic and outbreak cases in different areas in Guangdong province.