The
method of
monoclonal antibody-based
immunoassay has a great importance in the study of
quality control of
traditional Chinese medicine (TCM) and
detection of trace components in vivo
animals. Synthesis of small molecule artificial
antigen is the prerequisite for the establishment of this
method. In present study, catalpol-BSA was synthesized by
sodium periodate
oxidation method. Matrix-assisted
laser desorption ionization-
time-of-flight
mass spectrometry (
MALDI-TOF-MS) and molecular
exclusion chromatography showed that catalpol was successfully conjugated with BSA. The
mice could specifically produce anti-catalpol
antibodies with titer up to 18000. The artificial
antigen of catalpol was successfully synthesized.