<p><b>OBJECTIVE</b>To investigate the clinical and
molecular epidemiology characteristics of calicivirus and
adenovirus in
children for viral
diarrhea in Lanzhou.</p><p><b>
METHODS </b>Stool samples were collected from 295
children with
diarrhea at the First
Hospital of Lanzhou
University , Gansu Province,
China , between July 2010 and June 2011.
Reverse transcription -
polymerase chain reaction (RT-PCR) or
PCR were used to detected calicivirus and
adenovirus . The
adenovirus positive samples were typed by
nested PCR and multiple
PCR .</p><p><b>RESULTS</b>Of the 295 specimens, 13.2% (39/295) were positive for calicivirus, and 5.1% (15/295) were
adenovirus . Typing and
Phylogenetic analysis revealed that novirus GII-3 and
adenovirus 41 were the dominant
strains . Both calicivirus and
adenovirus predominately
affect children under the age of 2. In seasonal distribution, there was no obvious peak.</p><p><b>CONCLUSION</b>
Human calicivirus and
adenovirus are important pathogens of viral
diarrhea ,it is important to develop long-term systematic
surveillance .</p>