Preparation and identification of Jujuboside A artificial antigen / 中国中药杂志
Degeniee; Wei-Ku ZHANG; Hao-Yi QIAO; Hui-Hua QU; Yan ZHAO; Qing-Guo WANG; Jie-Kun XU.
China Journal of Chinese Materia Medica
; (24): 1880-1883, 2016.
Artículo
en Zh
| WPRIM | ID: wpr-250473
Immunogenic antigen (jujuboside A-BSA) and coating antigen (jujuboside A-OVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time. Jujuboside A artificial antigen was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunized mice were detected by enzyme-linked immunosorbent assay (ELISA). The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000. The jujuboside A artificial antigen was synthesized, which can be used further to preparation of monoclonal antibody and the pharmacokinetics study of jujuboside A in laboratory animals.
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