<p><b>OBJECTIVE</b>To introduce the eukaryotic expression vector pEGFP-C1-PDX-1 into
nestin-positive
cell derived from
bone marrow stromal cells by nucleofection and optimize the conditions for
transfection.</p><p><b>
METHODS</b>The recombinant
plasmid was transfected into
bone marrow stromal cells-derived
nestin-positive
cells with varied
DNA quantities or the
serum concentration in the medium. The expression of PDX-1
gene in the transfected
cells was detected by RT-PCR.</p><p><b>RESULTS</b>Satisfactory
efficiency of
transfection was achieved with the
DNA quantity of 2-10 microg and medium
serum concentration of 20%. PDX-1 expression was detected in the transfected
cells by RT-PCR.</p><p><b>CONCLUSION</b>The optimized
transfection conditions result in enhanced
efficiency of PDX-1
gene transfection into
nestin-positive
cells derived from
bone marrow stromal cells, which may serve as the
seed cells in
tissue-
engineering.</p>