The entrapped efficiency of BSA liposome / 药学学报
Dong-Zhi HOU; Chang-Ke LIU; Qi-Neng PING; Xiao-Hui LIANG; Dong-Zhi HOU; Chang-Ke LIU; Qi-Neng PING; Xiao-Hui LIANG.
Acta Pharmaceutica Sinica
; (12): 545-549, 2007.
Artículo
en Zh
| WPRIM | ID: wpr-268600
BSA liposomes were prepared with approximately 100 nm mean particle size under rather gentle experiment conditions, and two-colorimetric coomassie brilliant blue protein was employed to measure the free drug in the entrapped efficiency (EE%) determination of BSA liposomes. Gel filtration was used to measure the EE%, and several Sephadex gels were examined by the separation of liposomes and free drug. To determine the free drug, three methods were compared on two-colorimetric UV spectrophotography, Bradford and two-colorimetric coomassie brilliant blue, separately. Two-colorimetric coomassie brilliant blue process increased the accuracy and improved the sensitivity of the assay about 20-fold comparing with the Bradford method. Two-colorimetric coomassie brilliant blue assay appeared to be more sensitive and showed broader dynamic range to measure the free BSA in the EE% determination of BSA liposome.
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