<p><b>OBJECTIVE</b>To investigate the inhibitory effect of lentiviral vector-mediated
short hairpin RNA targeting
survivin (LV-
survivin shRNA ) on
angiogenesis and
growth of
endometriosis -like lesions in chick en
embryo chorioallantoic membrane .</p><p><b>
METHODS </b>Eutopic
endometrium from
women with
endometriosis was transplanted onto the non-vascular region of (CAM), where LV-
survivin shRNA was delivered subsequently. The
angiogenesis and the
growth of
endometriosis -like lesions in the CAM model were evaluated.</p><p><b>RESULTS</b>The
angiogenesis and formation of
endometriosis -like lesions were significantly suppressed in the CAM model by
treatment with LV-
survivin shRNA in comparison with those in the untreated CAM models and models treated with empty LV or DMEM (P<0.001). LV-
survivin shRNA also caused a significantly higher
cell apoptotic rate in the
endometriosis -like lesions than the other
treatments (P<0.001) and induced
necrosis in the lesions.</p><p><b>CONCLUSION</b>LV-
survivin shRNA can effectively inhibit
angiogenesis induced by the eutopic
endometrium and markedly suppress the formation of
endometriosis -like lesions in the CAM model.</p>