<p><b>OBJECTIVE</b>This study was aimed to investigate the
toxic effects of 3
nanomaterials, i.e. multi-walled
carbon nanotubes (MWCNTs),
graphene oxide (GO), and reduced
graphene oxide (RGO), on
zebrafish embryos.</p><p><b>
METHODS</b>The 2-h post-
fertilization (hpf)
zebrafish embryos were exposed to MWCNTs, GO, and RGO at different concentrations (1, 5, 10, 50, 100 mg/L) for 96 h. Afterwards, the effects of the 3 nanomateria on spontaneous
movement,
heart rate, hatching rate, length of
larvae,
mortality, and malformations ls were evaluated.</p><p><b>RESULTS</b>
Statistical analysis indicated that RGO significantly inhibited the hatching of
zebrafish embryos. Furthermore, RGO and MWCNTs decreased the length of the hatched
larvae at 96 hpf. No obvious morphological malformation or
mortality was observed in the
zebrafish embryos after exposure to the three
nanomaterials.</p><p><b>CONCLUSION</b>MWCNTs, GO, and RGO were all toxic to
zebrafish embryos to influence
embryos hatching and
larvae length. Although no obvious morphological malformation and
mortality were observed in exposed
zebrafish embryos, further studies on the
toxicity of the three
nanomaterials are still needed.</p>