<p><b>OBJECTIVE</b>To investigate the effect of
platelet-derived growth factor (
PDGF-BB) on the formation of
granulocyte-
monocyte colony forming unit (
CFU-GM) and
megakaryocyte colony forming unit (CFU-MK) and its anti-apoptotic effect on CHRF
cells.</p><p><b>
METHODS</b>The
CFU-GM and CFU-MK of murine and
human bone marrow cells were cultured
in vitro by using
plasma clot
culture system. The anti-apoptotic effect of
PDGF-BB on CHRF
cells and its mechanism were clarified by
flow cytometry.</p><p><b>RESULTS</b>
PDGF-BB 0-100 ng/ml stimulated the proliferation of murine and
human CFU-GM and CFU-MK in a
dose-dependent manner. The maximal stimulation effect was obtained at 50 ng/ml of
PDGF-BB (P<0.01). Furthermore,
PDGF-BB had an anti-apoptotic effect on CHRF
cells as shown by the
flow cytometry with AnnexinV/PI double
staining,
Caspase-3 expression and JC-1
detection (P<0.05).</p><p><b>CONCLUSION</b>
PDGF-BB significantly stimulates the proliferation of
CFU-GM and CFU-MK
in vitro, and has an anti-apoptotic effect on CHRF
cells.</p>