To compare the
amino acid metabolic profiling in
urine of
spontaneously hypertensive rats (SHR) and normal
Wistar rats, and investigate the regulatory effect of extract from
Coreopsis tinctoria on
blood pressure and
amino acid metabolic profiling in SHR. Right
aged SHR and
Wistar rats were housed to fit the new
environment for 2 weeks. After that, their
systolic pressure(SBP),
diastolic pressure(DBP) were measured and
urine was collected.
Amino acids profiles for SHR and
Wistar rats were acquired by using AQC precolumn derivatization
HPLC-
fluorescence method, and then partial
least squares discriminant analysis(PLS-DA) was applied to facilitate differentiation and determine metabolic differences between collected samples from two groups of
rats. Consequently, 40 SHR were randomly divided into 5 groups model group, high, middle, low
dosage groups of C. tinctoria extract (3.2, 1.6,0.8 g•kg⁻¹), and
captopril group (4 mg•kg⁻¹). They were treated for 4 weeks by ig
administration, and then their
urine samples were collected to determine the
amino acid metabolic profiling in various groups.
After treatment for 4 weeks, as compared with Wistar group,
serine,
alanine,
tyrosine, and
cystine in the
amino acid metabolic profiling were significantly increased in SHR group. As compared with SHR model group,
threonine and
methionine were decreased significantly in
captopril group (P<0.01);
amino acid metabolism was changed to different degrees in high, middle, and low
dosage groups of C. tinctoria extract, and the
threonine in low
dose group was significantly decreased (P<0.01);
serine and
threonine were decreased (P<0.05), and
valine,
methionine and
lysine were significantly decreased (P<0.01) in middle
dose group;
threonine,
valine,
methionine and
lysine were significantly decreased in large
dose group (P<0.01). The results showed that middle and high doses of extract from C. tinctoria could significantly improve disturbance of
amino acid metabolism, help to further clarify the
drug property
research of C. tinctoria, and provide data support for
amino acid metabolic pathway abnormalities in
hypertension patients.