<p><b>OBJECTIVE</b>To detect the expression of
survivin protein,
survivin mRNA, p27
protein, p27
mRNA and
PTEN protein in
hepatocellular carcinomas (HCC) and their clinical significances.</p><p><b>
METHODS</b>
Tissue microarrays were constructed. The expression of
survivin protein, p27
protein and
PTEN protein were evaluated by immunohistochemical
methods and in expression of
survivin mRNA and p27
mRNA were evaluated by in stiu hybridization respectively in
tumor tissues from 141 HCC
patients, 128 samples of para-
carcinoma liver tissues, 97
liver tissues far from the
carcinomas and normal
liver tissues from non HCC
patients. The relationship of
survivin, p27 and PTEN were investigated and a prediction model of HCC was constructed.</p><p><b>RESULTS</b>The expressions of
survivin protein (Ridit 95% CI = 0.689+/-0.048, P < 0.01),
survivin mRNA (Ridit 95% CI = 0.690+/-0.049, P < 0.01) and p27
protein (Ridit 95% CI = 0.556+/-0.053, P < 0.05) in HCC
tissues were significantly increased, while the expression of
PTEN protein (Ridit 95% CI = 0.282+/-0.048) in HCC
tissues was significantly reduced (P < 0.01).</p><p><b>CONCLUSION</b>Overexpressions of
survivin mRNA and p27
protein and reduced expression of
PTEN protein might be a valuable marker to predict the presence of HCC.</p>