Objective To evaluate the application of weak
cation exchange (WCX)
magnetic bead-based Matrix-Assisted
Laser Desorption Ionization
Time -of-Flight
Mass Spectrometry (
MALDI -TOF MS) in detecting differentially expressed
proteins in the
urine of renal clear
cell carcinoma (RCCC) and its value in the
early diagnosis of RCCC.
Methods Eleven newly diagnosed
patients (10
males and 1
female ,
aged 46-78, mean 63 years) of renal clear
cell carcinoma by
biopsy and 10
healthy volunteers (all
males ,
aged 25-32, mean 29.7 years) were enrolled in this study.
Urine samples of the RCCC
patients and healthy controls were collected in the morning. Weak
cation exchange (WCX) bead-based
MALDI -TOF MS
technique was applied in detecting differential
protein peaks in the
urine of RCCC. ClinProTools2.2
software was utilized to determine the characteristic
proteins in the
urine of RCCC
patients for the predictive model of RCCC. Results The
technique identified 160
protein peaks in the
urine that were different between RCCC
patients and
health controls; and among them, there was one peak (
molecular weight of 2221.71 Da) with statistical significance (P=0.0304). With genetic
algorithms and the
support vector machine , we screened out 13 characteristic
protein peaks for the predictive model. Conclusions The application of WCX
magnetic bead-based
MALDI -TOF MS in detecting differentially expressed
proteins in
urine may have potential value for the
early diagnosis of RCCC.