<p><b>OBJECTIVE</b>To establish a method for the determination of quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-D-gentiobioside in Semen Descurainiae.</p><p><b>METHOD</b>HPLC was used with self-made quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-D-gentiobioside as reference substances.</p><p><b>RESULT</b>The average collection was 99.78%, RSD 2.4%.</p><p><b>CONCLUSION</b>The method is appropriate for quality control of Semen Descurainiae.</p>